Three's a crowd to assess seed physiological quality for vegetable Brassica

3 Feb 25

Three’s a crowd to assess seed physiological quality for vegetable Brassica

The French-British-Japanese collaboration initiated through the VIGERM project supported by ISTA in 2019 continues to spread publications and methods.

A third article from the project was published at the end of last year in the journal Seed Science and Technology [1]. It concerns the early evaluation of germination and vigour of 4 vegetables from the Brassicaceae family: cabbage, radish, mustard and Chinese cabbage. It joins the two previous publications in the same journal [3,5] and highlights the trials carried out at GEVES during the training of a teacher from Tokyo University of Agriculture.

The comparative tests organised by GEVES in 2021 with 6 other volunteer laboratories from the ISTA Germination and Vigour Committees on 3 seed lots of cabbage, 2 lots of cauliflower and 5 lots of mustard resulted in a method validation for a radicle emergence test globally useable for vegetable Brassicas approved in 2024 by the ISTA Vigour Committee. The Technical Committee has therefore submitted to the Executive Committee a proposal for an amendment to the ISTA Rules which, if approved at the next general assembly, should lead to the addition of the method in 2026 Rules edition. In the meantime, the method validation report will soon be published in Seed Testing International, available on the ISTA website.

A total of three publications [1, 3, 5], two papers at the ISTA Symposium 2022 [2, 6] and one ISTA method validation report [4] have been produced by this international project.

References

Shinohara T., Ducournau S., Matthews S., Wagner M.-H. and Powell A. A., 2024. Manual and automated counts of early radicle emergence assess differences in seed vigour and normal germination in four vegetable Brassicaceae species. Seed Science and Technology, 52, 3, 327-342. https://doi.org/10.15258/sst.2024.52.3.09
Ducournau S., Shinohara T., Matthews S., Wagner M.-H., Powell A.A., 2022. RGB Image Analyses Assesses Radicle Emergence to Predict Normal Germination and Vigour in Brassica species. ISTA Seed Symposium, 02-04 November, Athens, Greece.
Shinohara T., Ducournau S., Matthews S., Wagner M.-H., and Powell A.A., 2021. Early counts of radicle emergence, counted manually and by image analysis, can reveal differences in the production of normal seedlings and the vigour of seed lots of cauliflower. Seed Science and Technology 49, 3, 219-235. https://doi.org/10.15258/sst.2021.49.3.04
Wagner M.-H., Ducournau S., Dupont A., Matthews S., Powell A.A., 2024. Application of an early count of radicle emergence at 20^oC to identify differences in vigour of Brassica spp. ISTA validation report, 5p.
Wagner M.-H., Powell A.A., Dupont A., Shinohara T., Ducournau S., 2023. Radicle emergence for cabbage can be assessed using multispectral imaging. Seed Science and Technology 51, 3, 291-296. https://doi.org/10.15258/sst.2023.51.3.01
Wagner M.-H., Dupont A., Matthews S., Powell A.A., Shinohara T., Ducournau S., 2022. Radicle emergence test can be assessed using multispectral imaging for Brassica oleracea. ISTA Seed Symposium, 02-04 November, Athens, Greece.